Basic planning for P2 laboratory engineering
I will explain to you the basic plan of laboratory engineering today:
I. Laboratory engineering equipment
1. Biosafety operating equipment (top or second level) needs to be set up and punctual inspection is required.
2. In order to dispose of the recombinant, and to use instruments such as grinders, freeze dryers, ultrasonic cell disrupters and centrifuges that generate aerosols, these instruments should be placed in safe operating equipment. However, if the machine already has equipment to avoid leakage of the aerosol, this is not the limit.
3, the laboratory needs to have autoclaves for the disposal of pollutants and waste sterilization, the best placed in the same room. If you have difficulty, you can place it in the vicinity.
4. The laboratory should be equipped with a UV lamp and a two-story door. If there is no two-story door, the door must be closed.
5. The laboratory should have equipment that can be used for foot or elbow operation or automatic hand washing.
Second, the laboratory project implementation requirements
1. When conducting the experiment, close the doors and windows of the laboratory.
2. Sterilize the test bench and safe operation equipment after the experiment is completed every day. If contamination occurs in the experiment, it should be sterilized immediately.
3. The waste of biological materials related to the experiment shall be sterilized and then discarded according to the method of “infectious waste disposalâ€. Contaminated utensils must be autoclaved before being cleaned or discarded. Do not use the mouth for suction operation.
4. Stop eating, smoking, and keeping food in the lab.
5. Wear gloves to prevent contamination when handling the recombinant. Wash hands or disinfect after handling and before leaving the laboratory. In all operations, avoid aerosols (for example, use hot white gold rings for inoculation). When inoculation into the culture medium, if many aerosols occur, it may constitute pollution). It should also be avoided to force the liquid in the straw or syringe to be forced out.
6. When removing contaminated items from the laboratory, they must be placed in a stable and leak-free container and sealed in the laboratory before being shipped out to prevent non-experimental organisms such as insects in the laboratory. And rodents, etc.
7. If other methods are available, avoid using a needle. In the laboratory, wear a lab coat and take off before disengagement to prevent people who do not know the nature of the experiment from entering the laboratory.
8. During the experiment, the “P2 level laboratory†should be marked in the laboratory and the “P2 level experiment in progress†should be marked. Moreover, the refrigerators and freezers that store the recombinants should be labeled as such. The laboratory should always be cleaned up and kept clean, and items not related to the experiment should not be placed.
9. HEPA filter in safe operation equipment, before the exchange, punctual inspection and experimental content changes, it is necessary to seal the safe operation equipment, fumigation with 10 grams of formaldehyde per cubic meter for 1 hour to remove pollution.
10. If the P1 level experiment is carried out together in this level of laboratory, it is necessary to clearly distinguish the experimental area and pay attention to the operation.
11. Follow other rules of the plan host.
Data reference (portal):
Air shower: http://
High efficiency air filter: http://
Clean room: http://
High efficiency filter leak detection: http://
Operating room purification project: http://
Cleanliness level standard: http://
Cleanroom testing standards: http://
Clean factory design specifications: http://
Cleanroom management system: http://
Clean room ventilation frequency standard: http://
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