Rna electrophoresis picture sharing and step introduction

China Security Network Xiaobian below introduces rna electrophoresis and rna electrophoresis picture sharing for big robbery

Reagents:

Agarose, formaldehyde, formamide, MOPS, EDTA, DEPC water.

step

Preparation of a 5 x MOPS-EDTA Buffer (0.1 M MOPS pH 7; 5 mM EDTA; 10 mM NaAc)

1. Weigh 20.9 g of MOPS and place in a 1 L beaker.

2. Add 700 mL of DEPC water to dissolve.

3. Adjust pH to 7.0 with 2N NaOH

4. Add 10 mL of 1M NaAC to the above solution.

5. Add 10 mL of 0.5 M EDTA pH=8.0 to the solution.

6. Add DEPC water to 1L

7. Filtered with 0.45um filter and stored in the dark

Preparation of trisaldehyde denaturing agarose gel Add 1 g of agarose to 31 mL of DEPC water, and add 10 mL of 5 x MOPS-EDTA Buffer to boil and dissolve. Add DOPC water to a volume of 41 mL. The solution was cooled to about 60 ° C, 9 mL of 37% formaldehyde was added, and poured into a glue tank and allowed to stand for 1 hour. (Add EB dye to the glue)

4. Electrophoresis Mix 1 x MOPS-EDTA Buffer electrophoresis solution in the electrophoresis tank, apply the sample, and electrophorese for 10 hours at 10v/cm. The electrophoresis solution in the electrophoresis tank was mixed once every 15 minutes during electrophoresis.